Our study has successfully verified that 99% or more of surface-associated bacteria *1on test pieces placed in a 1m3 test chamber might be eradicated when exposed to by SANYO’s electrolyzed mist*2 during the half-hour .

●Test method: A 20 µL bacterial solution of about 106CFU*3/mL in concentration was applied on the test pieces (76×26mm, made of slide glass). The test pieces were placed in a 1m3 test chamber with an air purification device equipped with an electrolyzed mist generator. After the door of the test chamber was tightly closed, the device was started, splaying electrolyzed mist. After the device operated for the given time period, the test pieces were removed from the chamber and the viable cell count was measured. The test was repeated in a similar manner with no mist splayed as a control test.
●Test bacteria: Staphylococcus aureus　NBRC 12732 (Staphylococcus aureus）
●Measuring method: Colony Titration Method.

*1:

The effectiveness of the device may depend on use conditions in the actual environment.

*2:

The diluted electrolyzed water created through the electrolyzation of tap water is discharged as a minute liquid drop in the air.

*3:

The unit used to represent the viable cell count, which is obtained by measuring the count of colonies associating on the solid medium, stands for Colony Forming Unit.

Electrolyzed water:
The electrolyzed water is made by electrolyzing tap water containing chloride ions, in other words, Cl negative ions, to generate 2 types of active oxygen, namely, hypochlorous acid and OH radical.
Electrolyzed mist:
The electrolyzed water is finely divided into smaller droplets by an ultrasonic transducer, and then diffused around a room together with a natural or fan-generated air flow.

20 µL bacterial solution of about 106CFU/mL in concentration was applied on the test pieces (76×26mm, made of slide glass). The test pieces were placed in a 1m3 test chamber with an air purification device equipped with an electrolyzed mist generator. After the door of the test chamber was tightly closed, the device was started, splaying electrolyzed mist (at the rate of about 80 mL/h) for a given time period. The test pieces were removed from the chamber and the viable cell count was measured. This test was repeated without device operation as a control test.
The viable cell count per test piece was: 30,000 CFU at the start of the test; and 41 CFU and less than 1 CFU (not detected) at the test points 30 and 90 minutes after the start of mist splaying, respectively. In contrast, the control test with no mist splayed showed that the viable cell count per test piece was 21,000 CFU and 5,000 CFU at the same test points, respectively. The comparison between these findings showed our electrolyzed mist has a 99% or higher eradication effectiveness after 30 minutes.

List of previously conducted research on the effects of electrolyzed water technology

Subject		Institution
Viral suppression	New H1N1 influenza	Gunma Prefectural Institute of Public Health and Environmental Sciences
	Influenza	Gunma Prefectural Institute of Public Health and Environmental Sciences
		Kitasato Institute Research Center for Medical Environment
		Kitasato Research Center of Environmental Sciences
	Avian influenza	Tottori University
	FCV (Norovirus)	Gunma Prefectural Institute of Public Health and Environmental Sciences
Bacterial elimination	Staphylococcus aureus and　　 Staphylococcus epidermidis	Gunma Prefectural Institute of Public Health and Environmental Sciences
		Kitasato Institute Research Center for Medical Environment
		Kitasato Research Center of Environmental Sciences
		Health Protection Agency　(HPA)　UK
		Japan Food Research Laboratories
		Chinese Center for Disease Control and Prevention, the People’s Republic of China
	E. coli, Bacillus subtilis, etc.	Food and Drug Safety Center
	General airborne bacteria	Kitasato Institute Research Center for Medical Environment
	eradicating surface-associated bacteria(Staphylococcus aureus)	Kitasato Institute Research Center for Medical Environment
Mold suppression	Black mold and Penicillium	Japan Food Research Laboratories
Odor elimination	Tobacco and pet odors	Internal assessment
Allergen suppression	Cedar pollen	Osaka Medical College
	Dust mites	Japan Environmental Sanitation Center
		Kobe University School of Medicine
		NPO Kenko Jutaku Fukyu Kyokai
	Dog and cat	Oriental Yeast Co., Ltd.
VOC elimination	Formaldehyde	Tsinghua University (China)

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